Karnataka Antibiotics & Pharmaceuticals Ltd., (KAPL) Bangalore is a Government Enterprise.  From a modest beginning in 1984, KAPL has grown from strength to strength in the areas of manufacturing and marketing of various life saving and essential drugs. With an ISO accredition from KPMG, Netherlands, KAPL is recognised for its total commitment to quality and services in domestic and international markets. On profit from inception, KAPL facilities are approved by many international clients.

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Health Management and Research Institute (HMRI) is a nonprofit organization working towards improved access and quality of healthcare services for all. HMRI is leveraging state-of-the-art information and communication technologies and modern management practices to transform healthcare delivery by creating the world’s largest integrated digital health network.
Piramal Healthcare Ltd, a Piramal Group company, is a globally integrated healthcare company that fulfills unmet medical needs across the world is integrated with HMRI
HMRI is in partnership with Maharashtra Government

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Punjabi University, Patiala, has kept up its spree of winning the highest "A" grade on a four-point scale amongst over 350 universities in the country. The status is awarded by the National Assessment and Accreditation Council (NAAC), which is an autonomous institution of the University Grants Commission, for a period of five years. The rare status is given by the NAAC on the basis of overall performance of a University during a space of five years.  Punjabi university was earlier given "five star level" by the NAAC for the period covering March 2002-2007.

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This Jan Aushadhi Campaign has already started with the opening of the first Jan aushadhi store at Amritsar on 25th November 2008 with the active participation of the CPSUs namely, IDPL,HAL,BPCL,KAPL & RDPL. With the launch of the Jan Aushadhi store at Shastri Bhavan, New Delhi, on 5th February, 2009, the campaign is reaching an ambitious phase of launching of more than 45 stores in the States of Andhra Pradesh, Delhi, Rajasthan, Maharashtra, Haryaa, Bihar, UP, Assam and Chattisgarh by March 2009.

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Karnataka Antibiotics & Pharmaceuticals Ltd., (KAPL) Bangalore is a Government Enterprise.  From a modest beginning in 1984, KAPL has grown from strength to strength in the areas of manufacturing and marketing of various life saving and essential drugs. With an ISO accredition from KPMG, Netherlands, KAPL is recognised for its total commitment to quality and services in domestic and international markets. On profit from inception, KAPL facilities are approved by many international clients.

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The Department of Biotechnology, Ministry of Science & Technology (Government of India) has established the Translational Health Science and Technology Institute (THSTI), an autonomous institution, as a part of the interdisciplinary Health Biotech Science Cluster, in the National Capital Region. The institution is designed to be a dynamic and intera ctive organization with a mission to conduct innovative translational research and develop research collaborations across disciplines and professions to accelerate the extension of concepts to the improvement of human health.

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Concepta is a Mumbai based independently owned full-service healthcare communications agency, aiming to provide innovative, original and credible content and communication. We have partnered with healthcare and pharmaceutical brands across the size spectrum, amongst multi-national as well as Indian organizations. Our expertise lies in developing print medical inputs: newsletters, product info. booklets, monographs, posters, patient education material, compendiums, etc. With a smart team of qualified professionals rich in experience, Concepta can be your trusted partner in growing your brands.

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About Authors:
Mona Karia*¹, Bhargav Gohel², Bina Thanki³, Darshan Madiya⁴, Shital Faldu^5
1,3M.Pharm, Smt. R.D.Gardi B.Pharmacy College. Rajkot, Gujarat, India
²Q.A. Officer, Intas Pharmaceutical Ltd., Ahemdabad.
⁴Assistant Professor of Smt. R.D.Gardi B.Pharmacy College, Rajkot
⁵Principal  of Smt. R.D.Gardi B.Pharmacy College, Rajkot
*mona.karia@yahoo.in

Abstract
An accurate, simple, precise and sensitive HPLC method with UV detection was developed and validated for simultaneous estimation of Etamsylate and Mefenamic acid in human plasma as per USFDA guidelines. Etamsylate and Mefenamic acid were spiked with human plasma and 5 % w/v Trichloro acetic acid used for protein precipitation in ration of 1:9 %v/v of human plasma. The column used was SUSEIDO C₁₈ column (250mm x 25 mm, i.d, 5 µ). Analysis was carried out with Methanol: ACN (70:30 % v/v): Buffer (0.05M Ammonium acetate buffer) pH 6.0 (85:15,% v/v) as mobile phase at flow rate of 1.5 ml/min at 298 nm with UV detection. The retention time of Mefenamic acid observed was 1.98 min and for Etamsylate 3.85 min respectively. The method was validated over the range of 0.5-30 μg/ml for Etamsylate and 0.2-18 µg/ml for Mefenamic acid with coefficients of regression 0.9939 and 0.9914 for Etamsylate and Mefenamic acid respectively. LOD and LOQ were 0.17 ng/ml and 0.51 ng/ml for Etamsylate and 2.62 ng/ml and 7.95 ng/ml for Mefenamic acid.The mean % recovery for MEF and ETM were found to be 89.07% and 88.98%with % C.V. of 1.87% and 1.91% respectively. Inter-day as well intra-day replicate, gave % RSD below 4.44 and 2.63 for Etamsylate and 3.20 and 3.75 for Mefenamic acid respectively. This RP-HPLC method is sensitive, selective, accurate, precise and rapid for the simultaneous estimation of Etamsylate and Mefenamic acid in human plasma with limit of Quantification in nano-gram level was developed and validated as per USFDA guideline and can be applied for a bioequivalence study to compare relative bioavailability of drug.

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ABOUT AUTHORS:
Sejal K. Patel, Sumeet I. Chhabra*
Department of Pharmaceutical Analysis,
Centre For Health Science Studies, Ganpat University,
Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India
*rxsumeet@gmail.com

ABSTRACT:
A simple, sensitive, accurate, precise and rapid reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of cefixime trihydrate and levofloxacin hemihydrate in tablet formulation. The chromatographic separation was performed on ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consisted of acetonitrile, water and methanol in the ratio of 65: 15: 25, v/v/v at a flow rate of 1.0 ml/min. The detection wavelength was set at 289 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the concentration range of 2-24 μg/ml for cefixime trihydrate and 2-30 μg/ml for levofloxacin hemihydrate. The retention times were found to be 1.9 ± 0.26 min for cefixime trihydrate and 3.6 ± 0.32 min for levofloxacin hemihydrate. The mean recoveries were 100.8 ± 0.54 and 100.1 ± 0.76 for cefixime trihydrate and levofloxacin hemihydrate, respectively. The method can be easily adopted for quality control analysis.

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